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  • Title: Reversibility of the insulin-stimulated phosphorylation of ATP citrate lyase and a cytoplasmic protein of subunit Mr 22000 in adipose tissue.
    Author: Belsham GJ, Brownsey RW, Denton RM.
    Journal: Biochem J; 1982 Apr 15; 204(1):345-52. PubMed ID: 7052067.
    Abstract:
    Exposure of rat epididymal adipose tissue to insulin leads to a 2-fold increase in incorporation of [32P]phosphate into ATP citrate lyase and a cytoplasmic protein of Mr 22000. A smaller increase and decrease in incorporation into cytoplasmic proteins of Mr 63000 and 20000 respectively were also observed. Subsequent addition of anti-insulin serum largely reversed these changes within 15 min, indicating that the alterations in 32P incorporation represented changes in net phosphate content of the proteins.
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