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  • Title: Cyanine dye measurement of a light-induced transient membrane potential associated with the metarhodopsin II intermediate in rod-outer-segment membranes.
    Author: Bennett N, Michel-Villaz M, Dupont Y.
    Journal: Eur J Biochem; 1980 Oct; 111(1):105-10. PubMed ID: 7053075.
    Abstract:
    The fluorescent dye 3,3'-dipropylthiodicarbocyanin iodide [diS-C3-(5)] is used as a probe of fast membrane potential variations in the study of rod-outer-segment membranes. The formation of metarhodopsin II is found to be associated with the generation of a transmembrane potential, which is dissipitated by ionic movements across the membrane; its risetime is very similar to that of the protonation of the protein, which is also associated with the formation of metarhodopsin II.
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