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  • Title: Dowex-1-induced reactions of reduced nicotinamide adenine dinucleotide (NADH).
    Author: Bernofsky C.
    Journal: Biochim Biophys Acta; 1982 Jan 12; 714(1):7-13. PubMed ID: 7055612.
    Abstract:
    Dowex 1-formate has been found to cause both anomerization and oxidation of NADH, and when NADH is chromatographed on a column of this resin, the major products observed are NAD+ and alpha-NAD+. Competing with the oxidation reaction is the conversion of NADH and alpha-NADH to unstable acid-modification products that subsequently break down during chromatography to give ADP-ribose and a variety of neutral and cationic degradation products. The effects of Dowex 1-formate on NADH differ from those of acid as oxidation is minimal when NADH is incubated in acid solution, although anomerization, acid-modification, and degradation to ADP-ribose and other products readily occur. The neural and cationic acid-degradation products that are formed from acid-modified NADH have been resolved by chromatography into 12 compounds, 6 of which react with 3-methyl-2-benzothiazolinone hydrazone and thus are identified as carbonyls. These substances gradually disappear from acid solution over a period of days and are replaced by polymeric pigments.
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