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  • Title: Enzymic heterogeneity of adrenocortical lysosomes: an X-ray microanalytical study.
    Author: Bácsy E.
    Journal: Histochem J; 1982 Jan; 14(1):99-112. PubMed ID: 7061238.
    Abstract:
    The enzymic heterogeneity of the lysosomal system has been demonstrated earlier. This study was concerned whether or not the lysosomes of the two outer zones of the rat adrenal cortex reveal any population characteristics on the basis of the activity of two enzymes. A double incubation method was used for the simultaneous electron cytochemical demonstration of acid phosphatase and arylsulphatase activity in the zonae glomerulosa and fasciculata of the adrenal. Lysosomes in semi-thin (0.5 micrometer) or ultra-thin sections were analysed with an ORTEC energy-dispersive X-ray microanalyser mounted on a JEOL TEMSCAN-100C electron microscope. Linearity of the amount of reaction deposit with incubation time was found for both enzymes. On the basis of the proportion of the two reaction products in individual lysosomes, three populations were distinguished. One with high acid-phosphatase and low, if any, arylsulphatase activity was only present in the zona glomerulosa. The other two populations exhibited stronger or weaker prevalence of arylsulphatase activity and were common in both zones. The values of the Ba/Pb ratio characteristic of each population changed with the reaction sequence but the principal distribution pattern did not. The nature of the interaction between the two reactions, as well as the possible functional significance of the lysosomal populations in the adrenal cortex, are discussed but are not yet clarified.
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