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  • Title: Evaluation of the Lumac kit for the detection of bacteriuria by bioluminescence.
    Author: Mackett D, Kessock-Philip S, Bascomb S, Easmon CS.
    Journal: J Clin Pathol; 1982 Jan; 35(1):107-10. PubMed ID: 7061712.
    Abstract:
    Four hundred and twenty-two urine samples were screened for significant bacteriuria using bioluminescence and microscopy of uncentrifuged urine. A smaller number of false-negatives were seen with bioluminescence (10%) than with microscopy (40%) while both techniques gave a similar number of false-positives (18%). The kit required a large amount of manual preparation, largely pipetting. With this and the short shelf-life of the reconstituted reagents, it is not suitable for small numbers of urines. At 45p per urine, the cost of bioluminescence is too high.
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