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  • Title: Renal sugar transport in the winter flounder. VI. Reabsorption of D-mannose.
    Author: Pritchard JB, Booz GW, Kleinzeller A.
    Journal: Am J Physiol; 1982 Apr; 242(4):F415-22. PubMed ID: 7065250.
    Abstract:
    The transport of D-mannose (Man) in flounder kidney was studied using renal clearance techniques in vivo and brush border membrane (BBM) vesicles in vitro. At plasma concentrations of 50-100 microM Man, the winter flounder (Pseudopleuronectes americanus) reabsorbed up to 70% of the filtered sugar. Man phosphates, but not free Man, accumulated in renal cells. Reabsorption of Man was reduced by phlorizin, D-glucose, methyl-alpha-D-glucoside, methyl-alpha-D-mannoside, and 2-deoxy-D-glucose. In BBM vesicles a Na+-dependent, phlorizin-sensitive overshoot in Man uptake (10 microM) was seen. Na+-dependent Man uptake was saturable, with an apparent Km of 127 microM. The transport properties for Man were identical in BBM vesicles from the winter flounder and southern flounder (Paralichthys lethostigma). The transport specificity was determined by cis-inhibition and trans-stimulation experiments with BBM. Glucose, galactose, 1,5-anhydro-D-mannitol (i.e., 1-deoxymannose), 2-deoxy-2-fluoro-D-glucose, and methyl-alpha-mannoside were shown to share the carrier-mediating mannose transport. 2-Deoxyglucose, methyl-alpha-2-deoxy-D-glucoside, and both the isomers (alpha and beta) of methyl-D-glucoside did not. In contrast, alpha-methyl-D-glucoside inhibited D-glucose transport both in vivo and in BBM vesicles. It is concluded that Man reabsorption in the flounder occurs via a Na+-cotransport system that also handles glucose but that differs from the glucose/methyl-alpha-D-glucoside reabsorptive pathway in that 1) an oxygen on C-1 is not required, and 2) an axial configuration for -OH on C-2 (C1 conformation) is readily accommodated.
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