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  • Title: Interactions of different albumins and animal sera with insolubilized Cibacron Blue, Evaluation of apparent affinity constants.
    Author: Naval J, Calvo M, Lampreave F, Piñeiro A.
    Journal: Comp Biochem Physiol B; 1982; 71(3):403-7. PubMed ID: 7067402.
    Abstract:
    1. A high concentration Cibacron Blue-Sepharose derivative has been used to study the affinity chromatography of albumin from eight animal species. 2. The apparent affinity constants for albumin varies between 3.9 x 10(4) M-1 and 0.9 x 10(4) M-1, in the order: Human greater than rabbit greater than horse greater than pig = dog greater than bovine greater than rat greater than chicken. 3. Other serum proteins were also bound to the gel, particularly lipoproteins and alpha 2-macroglobulin.
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