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  • Title: Cell surface carbohydrates in Crithidia deanei: influence of the endosymbiote.
    Author: Esteves MJ, Andrade AF, Angluster J, de Souza W, Mundim MH, Roitman I, Perreira ME.
    Journal: Eur J Cell Biol; 1982 Feb; 26(2):244-8. PubMed ID: 7067702.
    Abstract:
    The cell surface of the symbiote-containing and symbiote-free strains of Crithidia deanei were characterized comparatively by using 22 highly purified lectins with specificities for N-acetyl glucosamine, N-acetyl galactosamine, galactose, mannose-like residues, fucose and sialic acid. The specificity of the cell surface carbohydrate in both strains were analyzed by agglutination and lectin-binding assays. C. deanei with or without endosymbiote was specifically agglutinated by lectins from Triticum vulgaris (WGA) and Aaptos papillata suggesting the presence of D-GlcNAc residues. However, agglutination was stronger with the symbiote-free cells than with symbiote-containing organisms. The D-GalNAc-binding lectin from Wistaria floribunda also reacted most effectively with symbiote-free flagellates. Among D-Gal-binding lectins it was observed that those from Axinella polypoides and Ricinus communis I selectively agglutinated symbiote-free cells. In contrast the lectin from Arachys hypogaeae bound preferentially to the symbiote containing organisms. Both strains of C. deanei agglutinated strongly with the lectins concanavalin A and that from Lens culinaris (lectins for D-mannose-like residues). Conversely no cell agglutination occurred with the L-fucose-binding lectins Lotus tetragonolobus and Ulex europeus. The pattern of agglutination induced by the lectin from Limulus polyphemus of symbiote-free organisms was similar to that of symbiote-containing cells indicating the presence of sialic acid on the cell surface of both strains of C. deanei. These results indicate that the presence of the endosymbiote changes the lectin-binding sites at C. deanei surface membrane.
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