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  • Title: Ultrathinfilm immunoelectrophoresis.
    Author: Michiak A, Clark PM, Kricka LJ.
    Journal: Ann Clin Biochem; 1982 Mar; 19(Pt 2):114-6. PubMed ID: 7073212.
    Abstract:
    Immunoelectrophoresis (Laurell-rocket) in ultrathinfilm gels has been applied to the determination of human serum albumin in a model study. Agarose gels of 0.24 mm thickness were used and optimum electrophoretic conditions were determined. Precision was assessed and comparison with conventional immunoelectrophoresis and a chemical method was made. Though precision was poor (CV 4-6%) improvements in dispensing small volumes (0.2 microliter) should change this. Good correlation between methods (correlation coefficient 0.89-0.97) was obtained. The advantages of the technique are speed of analysis and low reagent costs.
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