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  • Title: Use of N-bromosuccinimide for the iodination of proteins for radioimmunoassay.
    Author: Reay P.
    Journal: Ann Clin Biochem; 1982 Mar; 19(Pt 2):129-33. PubMed ID: 7073216.
    Abstract:
    A new method, employing the mild oxidant N-bromosuccinimide, for the iodination of proteins for radioimmunoassay is described. Human prolactin, follicle-stimulating, luteinising, thyroid-stimulating, and growth hormones were iodinated with 125I by this method to specific activities up to 136 muCi/microgram. Incorporation of iodine ranged from 67% to 83% using 2 microgram hormone, 200 muCi Na125I, 0.5 nmol oxidant, and a reaction time of 15s. In comparative studies in immunological activity after iodination by this method was equal to, or greater than that by the chlorine gas, Iodo-Gen, or chloramine-T methods. The reagent is cheap and stable, and the method permits efficient use of antigen and iodide.
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