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  • Title: [Isolation, purification and properties of acid deoxyribonuclease from silkworm Bombyx mori eggs].
    Author: Bochkova AP, Filippovich IuB, Konichev AS.
    Journal: Biokhimiia; 1982 Mar; 47(3):489-96. PubMed ID: 7074175.
    Abstract:
    The acid deoxyribonuclease was isolated from Bombyx mori eggs and its physico-chemical properties were investigated. The enzyme purified 160-fold did not contain admixtures of phosphomono- and phosphodiesterases or ribonuclease. The molecular weight of the enzyme is 40 000 +/- 1000, isoelectric point lies at 6.5. The maximum activity is revealed at pH 5.2, 50 degrees. The DNAase is insignificantly activated by Mg2+ and is inhibited by Cu2+ and Zn2+. The enzyme preferentially hydrolyzes native DNA and is an endonuclease splitting DNA down to 5'-oligonucleotides.
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