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  • Title: DNA complementary to parathyroid mRNA directs synthesis of pre-proparathyroid hormone in a linked transcription-translation system.
    Author: Kronenberg HM, Roberts BE, Habener JF, Potts JT, Rich A.
    Journal: Nature; 1977 Jun 30; 267(5614):804-7. PubMed ID: 70756.
    Abstract:
    DNA complementary in sequence to the messenger RNA for pre-proparathyroid hormone was synthesised using reverse transcriptase. In a linked transcription-translation system using RNA polymerase and cell-free extract from wheat germ, the DNA directed the synthesis of a protein identified as pre-proparathyroid hormone by N-terminal sequencing and by electrophoretic and immunologic criteria.
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