These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Some characteristics of a urease-negative, temperature- sensitive strain of Bordetella bronchiseptica as a live, attenuated vaccine.
    Author: Shimizu T, Ishikawa H.
    Journal: Infect Immun; 1982 Apr; 36(1):198-201. PubMed ID: 7076293.
    Abstract:
    Strain ts-S34 of Bordetella bronchiseptica was treated jointly with two mutagens, nitrosoguanidine and UV irradiation, and a urease-negative (u-), temperature-sensitive (ts) mutant strain indistinguishable from Alcaligenes faecalis in its biochemical characteristics was isolated. However, the mutant isolated was a phase III organism. By repeating selection of smaller, hemolytic colonies observed among phase III colonies after prolonged incubation, a phase I organism, strain ts-S34.u-, was isolated. The u- and ts properties of the mutant strain were hereditarily stable, and heat-labile toxin production was very low. Growth in KCN broth and on Simmons citrate agar was reduced. Agglutinability of the strain against anti-S1 and anti-ts-S34.u- hyperimmune sera was as high (X20,480) as that of wild-type strain S1. The live ts-S34.u- strain vaccination protected guinea pigs from challenge exposure with 15,000 X the 50% lethal dose of virulent strain S1 of B. bronchiseptica. In these studies, it appeared that the ts-S34.u- strain has favorable properties, including a useful hereditary marker and low heat-labile toxin production, for use as a live attenuated vaccine.
    [Abstract] [Full Text] [Related] [New Search]