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  • Title: Routine measurement of phosphatidylglycerol in amniotic fluid.
    Author: Norman RJ, Joubert SM.
    Journal: S Afr Med J; 1982 May 29; 61(22):839-41. PubMed ID: 7079908.
    Abstract:
    Phosphatidylglycerol (PG) is a constituent of lung surfactant in the mature fetus and is effective in protecting against the respiratory distress syndrome. Three methods of separating PG by thin-layer chromatography have been assessed. A two-dimensional method, although separating the major phospholipids, is not suitable for routine use. One-dimensional resolution has the advantages of speed, duplication of samples and suitability of densitometric measurement of PG. Two methods are described and a technique suitable for the rapid demonstration of amniotic fluid PG is recommended.
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