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  • Title: Video time-lapse microscopy of phagocytosis and intracellular fate of crystalline nickel sulfide particles in cultured mammalian cells.
    Author: Evans RM, Davies PJ, Costa M.
    Journal: Cancer Res; 1982 Jul; 42(7):2729-35. PubMed ID: 7083163.
    Abstract:
    The endocytosis and intracellular distribution of carcinogenic crystalline nickel sulfide (NiS) particles in Chinese hamster ovary cells were studied using time-lapse video recording with phase-contrast and bright-field optics. Crystalline NiS particles were phagocytosedd by Chinese hamster ovary cells in regions of membrane ruffling. While these particles may remain bound to the cell surface for variable time intervals (min to hr), their internalization generally required only 7 to 10 min. Endocytosed crystalline NiS particles exhibited saltatory motion, and lysosomes were observed to interact repeatedly with the particles in a manner similar to that observed during the digestion of macropinosomes. Particles were never observed to be exocytosed from the cell, and with time, most of the internalized particles aggregated in the region around the nucleus. After 24 to 48 hr, particle saltation decreased to a point where the particle position became relatively fixed in the perinuclear region, and in some instances, this was associated with a conspicuous vacuole formation around the particles. It is concluded that the uptake and distribution of crystalline NiS particles occur by normal endocytic and saltatory processes as occur during the formation and breakdown of macropinosomes. The observed lysosomal interaction with phagocytoses cytoplasmic NiS may accelerate particulate nickel dissolution allowing entry of ionic nickel into the nucleus.
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