These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Dopaminergic receptors in cat brain. Action of triton X-100 on [3H]spiroperidol binding and localization in relation to the synaptic region.
    Author: Jimenez Aguilar C, De Robertis E.
    Journal: Eur J Pharmacol; 1982 Apr 08; 79(1-2):1-9. PubMed ID: 7084300.
    Abstract:
    [3H]Spiroperidol binding was used to assay dopaminergic receptors in synaptosomal membranes isolated from basal ganglia of cat brain. In addition to the usual filtration, a special centrifugation technique was developed which has some advantages for ligands with high non-specific binding. The specific binding to control membranes showed a high affinity binding site with KD 0.99 nM and Bmax 445 fmol/mg protein, both determined by Scatchard analysis. Addition of 10(-5) -10(-3)% Triton X-100 increased the Bmax of the high affinity binding site without a change in KD. With higher concentrations of detergent (10(-2) -10 (-1)% there was inhibition of binding. Both the activation and the inhibition of binding were reversed by washing of the detergent; but the reversal of the activation was accompanied by a decrease in affinity. The binding to synaptosomal membranes treated with 10(-1) - 10(-2)% Triton X-100 and then washed led to a loss of receptors probably present in the presynaptic membrane. These and other results suggest that dopaminergic receptors are localized both pre- and postsynaptically in relation to the synaptic region.
    [Abstract] [Full Text] [Related] [New Search]