These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Purification and macromolecular properties of a sialic acid-specific lectin from the slug Limax flavus.
    Author: Miller RL, Collawn JF, Fish WW.
    Journal: J Biol Chem; 1982 Jul 10; 257(13):7574-80. PubMed ID: 7085639.
    Abstract:
    A lectin (LFA) which is highly specific for sialic acid has been purified from the slug Limax flavus by a combination of ammonium sulfate fractionation and affinity chromatography on bovine submaxillary mucin coupled to Sepharose 4B. The affinity-purified lectin appeared homogeneous by electrophoresis in the presence of sodium dodecyl sulfate. Below 1 mg/ml at pH 7, LFA exists as a species of Mr = 44,000 which is composed of two equal sized subunits. Above 1 mg/ml, the protein solution was observed to behave as a rapidly associating-dissociating system. N-acetylneuraminic acid and N-glycolylneuraminic acid gave a 50% inhibition of agglutination of erythrocytes by LFA at 0.13 and 0.81 mM, respectively. Galactose, N-acetylgalactosamine, galactosamine, glucose, N-acetylglucosamine, glucosamine, mannose, arabinose, xylose, fucose, glucuronic acid, alpha-methyl-D-glucoside, alpha-methyl-D-mannoside, lactose, and sucrose were ineffective inhibitors at concentrations up to 10-25 mM. Bovine submaxillary mucin, a sialoprotein, was a potent inhibitor of hemagglutination by LFA. Upon treatment of the mucin with neuraminidase, loss of inhibitory activity was observed which was proportional to the loss of sialic acid from the mucin.
    [Abstract] [Full Text] [Related] [New Search]