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  • Title: Cryopreparation of mammalian tissue for X-ray microanalysis in STEM.
    Author: Zierold K.
    Journal: J Microsc; 1982 Feb; 125(Pt 2):149-56. PubMed ID: 7086879.
    Abstract:
    A cryopreparation technique for studies of ultrastructure and distribution of diffusible elements in biological tissue is described. Electron microscopical contrast and characteristic X-ray spectra are found to be poor in completely frozen-hydrated ultrathin cryosections of fresh chemically untreated tissue. Both STEM contrast and detection of characteristic X-rays are enhanced by careful freeze-drying in the microscope. Although the ultrastructure is affected by ice crystals, intracellular compartments can be identified by STEM without staining and studied by X-ray microanalysis.
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