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  • Title: A simple automated colorimetric method for determination of N-acetyl- beta-D-glucosaminidase.
    Author: Moore JC, Morris JE.
    Journal: Ann Clin Biochem; 1982 May; 19(3):157-9. PubMed ID: 7092140.
    Abstract:
    An automated method for the determination of N-acetyl-beta-D-glucosaminidase in serum or plasma using p-nitrophenol (pNP) N-acetyl-beta-D-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 +/- 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variation 1.2% and inter-assay coefficient of variation 1.6%. Inhibition of enzyme activity by heparin in plasma samples can be reversed by the addition of calcium chloride to the buffer.
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