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  • Title: [Percentage of monocytes in preparations of mononuclear cells from normal subjects: evaluation by a new rapid method for the identification of esterase-positive cells].
    Author: Nardiello S, Pizzella T, Russo M, Galanti B.
    Journal: Boll Soc Ital Biol Sper; 1982 Apr 30; 58(8):467-70. PubMed ID: 7093054.
    Abstract:
    A rapid suspension method for alpha-naphthyl acetate esterase staining has been utilized to evaluate monocytes percentages in Ficoll-Paque separated mononuclear cells (MC) from normal individuals. In MC preparations from 24 normal subjects a mean percentage of 8% monocytes has been found, with a range from 2% to 20%. Cell fractions depleted of T-lymphocytes by rosette sedimentation showed 43% of esterase positive cells (range 31-53%), while very few monocytes (2%) appeared to contaminate the preparations of E-rosetting cells. The method for esterase staining of monocytes while they are in suspension is very simple and permits a rapid and accurate evaluation of monocyte percentages in mixed cell preparations.
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