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  • Title: Analysis of picomolar concentrations of 6-oxo-prostaglandin F1 alpha in biological fluids.
    Author: Barrow SE, Waddell KA, Ennis M, Dollery CT, Blair IA.
    Journal: J Chromatogr; 1982 Apr 30; 239():71-80. PubMed ID: 7096507.
    Abstract:
    A highly sensitive and specific assay for the quantitation of 6-oxo-prostaglandin F1 alpha, the stable hydrolysis product of prostacyclin, is described. The method involves the addition of [3,3',4,4'-2H4]-6-oxo-prostaglandin F1 alpha as internal standard, extraction from biological fluids using muBondapak C18 reversed-phase Sep-Paks, and preliminary purification by normal-phase chromatography. Following conversion to the methoxime, tris-trimethylsilyl, pentafluorobenzyl derivative, samples were analysed using combined capillary column gas chromatography negative ion chemical ionisation mass spectrometry. Fragments ions at m/z 614 (1H) and 618 (2H) [M-C7H2F5]- were monitored for quantitation. This method was used for the measurement of endogenous levels of 6-oxo-prostaglandin F1 alpha in human urine and for the determination of prostacyclin release from rat peritoneal mast cells and from rat aortic rings incubated in human plasma.
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