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  • Title: An automated HPLC assay for simultaneous quantitation of methylated xanthines and uric acids in urine.
    Author: Tang-Liu DD, Riegelman S.
    Journal: J Chromatogr Sci; 1982 Apr; 20(4):155-9. PubMed ID: 7096523.
    Abstract:
    To investigate the elimination kinetics of caffeine and its metabolites, as well as the interaction between them, an automated HPLC method is described. This method involves a single extraction procedure, followed by a gradient elution. Fourteen methylated xanthines and uric acids are well separated with an assay sensitivity of 1 microgram/ml when one-half ml of urine is used. The assay is highly selective, from endogenous compounds, and reproducible. This method is recommended for accurate pharmacokinetic studies.
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