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  • Title: Continuous-flow analysis for serum albumin, by use of the immediate reaction with bromcresol green.
    Author: Plesher CJ.
    Journal: Clin Chem; 1978 Nov; 24(11):2036-9. PubMed ID: 709843.
    Abstract:
    Albumin reacts with bromcresol green reagent almost immediately, other serum proteins more slowly. This phenomenon can be exploited to improve the specificity of this routine method for determining serum albumin. I describe a 30-s reaction as adapted to continuous-flow analyzers, with no interference from other serum proteins. The method shows linearity to 72 g/liter. Results of this method correlate very well with those by the Laurell "rocket" and manual (30-s) bromcresol green methods. This procedure gives a considerable improvement in specificity for continuous-flow analyzers as compared with current bromcresol green methods.
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