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  • Title: Direct transfer of one-carbon units in the transformylations of de novo purine biosynthesis.
    Author: Smith GK, Mueller WT, Slieker LJ, DeBrosse CW, Benkovic SJ.
    Journal: Biochemistry; 1982 Jun 08; 21(12):2870-4. PubMed ID: 7104299.
    Abstract:
    It is shown that the transfer of formyl units in the de novo purine biosynthetic pathway as catalyzed by glycinamide ribonucleotide (GAR) transformylase and 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) transformylase probably proceeds through a direct displacement mechanism involving only formyl donor (10-CHO-H4folate) and formyl acceptor (GAR or AICAR). The inability to observe enzyme-catalyzed solvent oxygen incorporation or uncoupling by hydroxylamine of 1:1 stoichiometry between formylated acceptor [formylglycinamide ribonucleotide or 5-(formylamino)imidazole-4-carboxamide ribonucleotide] and deformylated donor implies the absence of an amidine intermediate and suggests that either a formylated enzyme-bound intermediate is not formed or such an intermediate is not accessible to hydroxylamine.
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