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  • Title: The alkaline hydrolysis of phosphotriesters in alkylated mammalian DNA.
    Author: Crathorn AR, Shooter KV.
    Journal: Biochim Biophys Acta; 1982 May 31; 697(2):259-61. PubMed ID: 7104358.
    Abstract:
    Isolated DNA was alkylated with N-[14C]methyl-N-nitrosourea or N-[14C]ethyl-N-nitrosourea. Sedimentation analysis of the alkylated DNA before and after alkaline hydrolysis was used to determine the number of single-strand breaks introduced by hydrolysis of the triesters. Vacuum distillation from alkylated DNA solutions before and after alkaline hydrolysis was used to determine the numbers of triesters hydrolysing to the alcohol.
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