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  • Title: A simple reproducible method for prometaphase chromosome analysis.
    Author: Rybak J, Tharapel A, Robinett S, Garcia M, Mankinen C, Freeman M.
    Journal: Hum Genet; 1982; 60(4):328-33. PubMed ID: 7106769.
    Abstract:
    A method is described for the analysis of chromosomes in prophase and early metaphase. It involves culturing the lymphocytes in medium RPMI-1640, supplemented with 10% autologous plasma instead of fetal bovine serum. Living cells are treated with actinomycin D and colcemid for 1 h prior to harvest and harvested early at 65 h of incubation, using a hypotonic solution formulated by Ohnuki (1968). The method has been tested on several hundred clinical samples on a routine basis. On average, 30% of the dividing cells were in prometaphase.
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