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  • Title: Determination of the antibody to hepatitis B e antigen by the inhibition on electrophoresis of free e antigen.
    Author: Gotanda T, Imai M, Sano T, Oinuma A, Nomura M, Miyakawa Y, Mayumi M.
    Journal: J Immunol Methods; 1982; 51(3):347-53. PubMed ID: 7108234.
    Abstract:
    A new method is described for the rapid and sensitive detection of the antibody to hepatitis B e antigen (anti-HBe) in serum. Twenty-five microliters of the test serum were incubated with 10 microliter of the purified small 'free' e antigen, and then the consumption of the added antigen was detected by the failure to precipitate with standard anti-HBe in counter-electrophoresis. When a total of 444 serums from asymptomatic carriers of hepatitis B surface antigen was tested for anti-HBe by this method, 348 (78.4%) were found to be positive with a detectability much higher than that of the passive hemagglutination method which detected the antibody in only 264 (59.5%) of the same serums.
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