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  • Title: [Method of plasma acetate determination by gas chromatography after ethanol administration in man (author's transl)].
    Author: Bruno R, Santoni Y, Botta A, Cano JP.
    Journal: Toxicol Eur Res; 1982 Jan; 4(1):31-4. PubMed ID: 7112562.
    Abstract:
    Plasma acetate is extracted using ethanol in alkaline medium, in presence of internal standard. After concentration of the hydroalcoholic phase, the chromatographic analysis is performed utilizing 10% SP 1200 and 1% H3PO4 on chromosorb W. The linearity of the method has been tested from 0.05 to 10 mM/I, its reproducibility is +/- 3.5% for levels ranging from 0.1 to 10 mM/I and +/- 10% for those smaller than 0.1 mM/I. This method is sensitive enough to assess the physiological acetate levels and those obtained after ethanol biotransformation.
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