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  • Title: Variation in mitogen-induced ovine lymphocyte blastogenesis: adherent cells or 2-mercaptoethanol restore randomly depressed responses.
    Author: Fiscus SA, DeMartini JC, Pearson LD.
    Journal: Vet Immunol Immunopathol; 1982 May; 3(3):345-59. PubMed ID: 7112915.
    Abstract:
    Peripheral blood leucocytes (PBL) isolated 23 times over a 6-week period from four normal sheep showed considerable variation in serially tested responses to predetermined optimal concentrations of concanavalin A (Con A), phytohaemagglutinin (PHA), and pokeweed mitogen (PWM). Con A responses, in particular, varied widely and were often randomly depressed (21 of 91 times compared to 15 of 91 times for PHA or PWM). The addition of as few as 1% adherent cells (AC) to depressed cultures fully restored the PBL proliferative response to normal levels. Addition of greater numbers of AC (5 or 10%) had little further enhancing effect on depressed cultures. The addition of 1, 5, or 10% AC to cultures that were responding at normal levels increased responses only slightly. Autologous or allogeneic AC were equally effective. Addition of 2-mercaptoethanol (2-ME) to depressed cultures only partially restored the blastogenic response to Con A and had little effect on normal cultures.
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