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  • Title: Radioimmunoassay for the antifreeze polypeptides of the winter flounder: seasonal profile and immunological cross-reactivity with other fish antifreezes.
    Author: Slaughter D, Hew CL.
    Journal: Can J Biochem; 1982 Aug; 60(8):824-9. PubMed ID: 7127188.
    Abstract:
    The polymerization of winter flounder antifreeze polypeptide (AFP) with glutaraldehyde to increase its antigenicity and the conjugation of the AFP to bovine serum albumin so that the AFP could carry a radioiodine label have allowed the development of a convenient and reliable radioimmunoassay (RIA) for the antifreeze. A seasonal concentration profile of serum AFP as determined by the RIA corresponded closely with its activity profile. Winter serum concentrations of AFP measured by the RIA (7-11 mg/mL) agreed well with antifreeze recovery from Sephadex G-75 chromatography of serum (6-10 mg/mL), but less so with concentration estimates reported from activity measurements (12-25 mg/mL) (Petzel, D. H., Reisman, H. M. & DeVries, A. L. (1980) J. Exp. Zool. 211, 63-69). A small but measurable immunological cross-reactivity was detected between the antiflounder AFP and other fish antifreeze polypeptides. Such cross-reactivity was quite unexpected considering the large compositional and structural differences among the various AFP and its significance remains unclear.
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