These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Characterization of a human melanoma-associated ganglioside antigen defined by a monoclonal antibody, 4.2.
    Author: Nudelman E, Hakomori S, Kannagi R, Levery S, Yeh MY, Hellström KE, Hellström I.
    Journal: J Biol Chem; 1982 Nov 10; 257(21):12752-6. PubMed ID: 7130176.
    Abstract:
    A monoclonal IgM antibody, 4.2, reacting with a surface antigen expressed on most human melanomas (Yeh, M.-I., Hellström, I., Abe, K., Hakomori, S., and Hellström, K. E. (1982) Int. J. Cancer, 29, 269-275) was shown to be directed to a ganglioside with the carbohydrate structure NeuAc alpha 2 leads to 8NeuAc alpha 2 leads to 3Gal beta 1 leads to 4Glc beta 1 leads to 1Cer. The carbohydrate structure, established by enzymatic degradation and methylation analysis with mass spectrometry, is identical with that of brain GD3 ganglioside, but its ceramide is characterized by a predominance of longer chain fatty acids, in contrast to brain GD3 that has mainly C18:0 fatty acid. The antibody did not react with various other gangliosides, including those having the same terminal sugar sequence (NeuAc alpha 2 leads to 8NeuAc alpha 2 leads to 3Gal) as GD3, such as GT1a and GQ1b. Its specificity is therefore restricted to the NeuAc alpha 2 leads to 8NeuAc alpha 2 leads to 3Gal beta 1 leads to 4Glc leads to Cer sequence, including the innermost sugar, Glc residue.
    [Abstract] [Full Text] [Related] [New Search]