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  • Title: Fluorescence anisotropy of labelled F-actin. Influence of Ca2+ on the flexibility of F-actin.
    Author: Miki M, Wahl P, Auchet JC.
    Journal: Biophys Chem; 1982 Oct; 16(2):165-72. PubMed ID: 7139050.
    Abstract:
    We measured the fluorescence static anisotropy and the time-resolved fluorescence anisotropy decay of F-actin labelled with N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine at 20 degrees C in solutions containing 100 mM KCl and free Ca2+ at various concentrations. The average fluorescence anisotropy and the fluorescence rotational correlation time of actin decreased in the presence of micromolar concentrations of free Ca2+. The change of the rotational correlation time of labelled actin could not be explained by a variation of the actin critical concentration. We concluded therefore that F-actin undergoes a conformational change induced by Ca2+ binding. The binding constant was 6 X 10(6) M-1.
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