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  • Title: [Horseradish peroxidase: reagent for stopping the catalytic conversion of the substrates H2O2 and 2,2'-azino-di(3-ethyl-benzathiazoline-sulphonic acid-(6)) (ABTS)].
    Author: Gallati H, Brodbeck H.
    Journal: J Clin Chem Clin Biochem; 1982 Oct; 20(10):757-60. PubMed ID: 7142914.
    Abstract:
    Catalytic conversion of H2O2 and 2,2'-azino-di(3-ethyl-benzthiazoline-sulphonic acid-(6)) (ABTS) by peroxidase is stopped immediately and completely by the addition of a secondary alkyl sulphate (Teepol-610) or sodium dodecyl hydrogensulphate. The resulting colour intensity of the oxidized ABTS+ is thereby stabilized and can be determined photometrically within 60 min. These stop reagents have special practical importance for the enzyme-(peroxidase)-immunological determination by the ELISA technique; but the activity of the indicator enzyme must be measured by the two point method, owing to the heterogeneity of the test system and the slow reaction rate.
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