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  • Title: A new hexasaccharide chain isolated from bovine colostrum kappa-casein taken at the time of parturition.
    Author: Saito T, Itoh T, Adachi S, Suzuki T, Usui T.
    Journal: Biochim Biophys Acta; 1982 Nov 24; 719(2):309-17. PubMed ID: 7150644.
    Abstract:
    The carbohydrate units linked to caseinoglycopeptide from colostrum taken 30 min after parturition were released as reduced oligosaccharides by alkaline borohydride treatment, and separated into four acidic oligosacchariditols (a hexa- (11.0%), penta- (38.5%), tetra- (35.5%) and a trisacchariditol (15.0%)). Structural studies showed that the hexasacchariditol was a new structure and had the chemical structure: NeuNAc-alpha-2-3-Gal-beta-1-3-[NeuNAc-alpha-2-3-Gal- beta-1-4-GlcNAc-beta-1-6-]-N-acetylgalactosaminitol(GalNAcItol). The other three oligosacchariditols were shown to be NeuNAc-alpha-2-3-Gal-beta-1-3-[Gal-beta-1-4-GlcNAc-beta-1-6-]-GalNAcItol, NeuNAc-alpha-2-3-Gal-beta-1-3-[NeuNAc-alpha-2-6-]-GalNAcItol and NeuNAc-alpha-2-3-Gal-beta-1-3-GalNAcItol, which were identical with those found previously in colostrum kappa-casein taken 6 h after parturition.
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