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  • Title: Microspectrophotometric studies on the visual pigment in the intact retina of the goldfish, Carassius auratus (Linn.).
    Author: Zyznar ES, Larson WL, Ali MA.
    Journal: Rev Can Biol; 1978 Sep; 37(3):143-56. PubMed ID: 715263.
    Abstract:
    The visual pigments in intact retinas of the common goldfish were examined by microspectrophotometry. Difference spectra free of photoproduct contamination had absorbance maxima at lambda527 nm +/- 2 nm. The average pigment density, about 0.32, was almost invariant with regard to retinal location and specimen size. Traces of a photoproduct absorbing maximally at lambda480-490 nm were found. The normalized "absorbance" curve deviated from standard templates. It was suggested that this was an effect of cross-coupling of energy and among photoreceptors. Finally, known action spectra show a red shift relative to the absorbance spectra measured.
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