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  • Title: Improved method for routine determination of nicotine and its main metabolites in biological fluids.
    Author: Stehlik G, Kainzbauer J, Tausch H, Richter O.
    Journal: J Chromatogr; 1982 Nov 12; 232(2):295-303. PubMed ID: 7153279.
    Abstract:
    ExtrelutR extraction and glass capillary gas chromatography were applied to the routine determination of nicotine and its metabolites cotinine, nicotine-1'-N-oxide and cotinine-1-N-oxide in urine and plasma. After extraction of nicotine and cotinine both N-oxides and phendimetrazine-N-oxide (used as internal standard) were reduced to their bases by SO2 on-column and eluted by a mixture of diethyl ether and dichloromethane. The minimum detectable concentrations are 0.03 micrograms/ml for urinary nicotine and cotinine and 0.1 microgram/ml for the N-oxides. In plasma samples the corresponding values are 5 ng/ml and 15 ng/ml, respectively, with sample values as small as 2 ml. The advantage of the direct determination of all four compounds of interest in one sample reduced the amount of plasma required. The straightforward and rapid extraction and reduction procedure as well as the long-term stability of the gas chromatographic separation system make the method suitable for routine application.
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