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  • Title: [Comparative morphological study of erythrocytes in various types of whole blood and packed cell preparations stored in liquid state].
    Author: Cazin P, Fabre G, Allary M, Saint-Blancard J.
    Journal: Rev Fr Transfus Immunohematol; 1982 Oct; 25(5):499-512. PubMed ID: 7163730.
    Abstract:
    Morphological comparative study observed by scanning electron microscopy have been made on three blood stored in PVC plastic bags at 4 degrees C: 1. Whole blood in CPD, CPD-A,D (CPD added with 0,4 mM adenine and enriched with glucose 1,5 time the normal concentration) and ACD-Ad,Gua (ACD with 0,5 mM adenine and 0,5 mM guanosine); 2. Red blood cells obtained by centrifugation from blood collected in CPD, CPD-A,D and ACD-Ad, Gua; 3. Red blood cells from blood collected in CPD or ACD and resuspended in balanced, nutritious and enriched media: SAG (Saline, Adenine, Glucose) or PAGGS (Phosphate, Adenine, Guanosine, Glucose, Saline) (CPD/SAG, ACD and CPD/PAGGS). The results of statistical analysis show that the addition of adenine and guanosine in the preservative solutions improves the morphological quality of erythrocytes. On this view the resuspended media are the least traumatic. For example, in the concentrates CPD/PAGGS the level of normal or practically shape cells (discocytes and echinocytes I) nears 70 p. cent at the 6th week of storage. Thus the PAGGS system appears superior to SAG system.
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