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  • Title: Quantitative separation of estrogens, androgens and progestogens using reverse phase high pressure liquid chromatography.
    Author: Kling OR, diZerega GS.
    Journal: Steroids; 1982 Oct; 40(4):381-8. PubMed ID: 7170749.
    Abstract:
    Dual UV wavelength scanning at 206 and 254 nm was used to develop a sensitive, separation and quantitation procedure for estrone, estradiol-17 beta, testosterone, 4-androstene-3,17,-dione, progesterone and 17-hydroxyprogesterone using reverse phase high pressure liquid chromatography. The difference in UV absorption of the estrogens from the androgens and progestogens allows for correction of the co-elution of testosterone with estradiol-17 beta and 4-androsten-3,17,-dione with estrone. Incorporation of an isocratic step-gradient provides improved separation while maintaining shortened elution times for the less polar steroids.
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