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  • Title: [Methods and their evaluation in estimating the vitamin B6-status in humans. 3. Determination- of 4-pyridoxic acid in urine].
    Author: Simon I, Leinert J, Hötzel D.
    Journal: Int J Vitam Nutr Res; 1982; 52(3):280-6. PubMed ID: 7174226.
    Abstract:
    A method based on the fluorimetric determination of the 4-PA-lactone is described. The applied method saves time and work compared to others. The analytical procedure may be interrupted for a few days after eluting the 4-PA from the ion exchanger as well as before measuring the transmission. Accuracy, precision and sensitivity of the method are demonstrated. Interfering substances influencing the fluorescence measurement and probable errors caused by the reference value creatinine are discussed. Storage tests applied with the urine samples at--18 degrees C have shown that 4-PA-values do not change considerably within two months.
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