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  • Title: Quantitation of sulfhydryl groups on erythrocytes in polycythemia vera.
    Author: Hougland MW, Luethke JM.
    Journal: Histochemistry; 1982; 76(1):57-60. PubMed ID: 7174367.
    Abstract:
    The number of sulfhydryl groups on the surface of intact erythrocytes from patients with polycythemia vera is significantly less (34%) than those from control subjects. Sulfhydryl groups were measured indirectly by reversibly blocking -SH groups with the thiol reagent, 6,6'-dithiodinicotinic acid, which forms stable mixed disulfides on the surface of erythrocytes. Glutathione was used to break the disulfides and release thione into the supernatant. Thione was then quantitated spectrophotometrically.
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