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  • Title: Zinc-dependent synthesis of 5',5'-diadenosine tetraphosphate by sheep liver lysyl- and phenylalanyl-tRNA synthetases.
    Author: Brevet A, Plateau P, Cirakoğlu B, Pailliez JP, Blanquet S.
    Journal: J Biol Chem; 1982 Dec 25; 257(24):14613-5. PubMed ID: 7174656.
    Abstract:
    Zinc greatly stimulates the initial rate of in vitro synthesis of 5',5'-diadenosine tetraphosphate by sheep liver lysyl- and phenylalanyl-tRNMA synthetases. In the case of each enzyme, maximum stimulation of 50- to 100-fold is reached upon addition of 50 microM ZnC2 to a reaction mixture (37 degrees c, pH 7.8) containing 50 microM dithioerythritol, 150 mM KCl, 5 mM ATP, 10 mM MgCl2, 0.1 mM cognate L-aminoacid, catalytic amounts of the aminoacyl-tRNA synthetase, and unlimiting pyrophosphatase activity. This observation made with aminoacyl-tRNA synthetases of mammalian origin supports the proposal that changes in cellular free zinc ion concentration could contribute to 5',5'-diadenosine tetraphosphate variations in animal cells as a function of growth activity.
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