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  • Title: Quantification of Schistosoma mansoni in mouse lungs by radioassay and autoradiography of 75Se-labeled schistosomula.
    Author: Georgi JR, Dean DA, Chandiwanna SK.
    Journal: J Parasitol; 1982 Dec; 68(6):1092-5. PubMed ID: 7175612.
    Abstract:
    Lung radioassay and compressed lung autoradiography of 75Se-labeled schistosomula were used to quantify lung schistosomula. Recovery of schistosomula from minced lung tissue after a 4-hr incubation yielded 69.4 +/- 6.3% of the total number present as demonstrated by subsequent autoradiography of these tissues. Radioassay of lung tissue was considered a valid alternative to autoradiography only for parallel comparisons and those not requiring estimation of the actual number of schistosomula. The number of schistosomula in the lungs on day 6 was significantly correlated with radioactivity in the tail on day 1 (r2 = 91.8%). It was concluded that compressed lung autoradiography provides both an alternative to and an adjunct to standard schistosomulum recovery techniques in the assay of early S. mansoni infections.
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