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  • Title: Growth of Babesia bovis in bovine erythrocyte cultures.
    Author: Erp EE, Gravely SM, Smith RD, Ristic M, Osorno BM, Carson CA.
    Journal: Am J Trop Med Hyg; 1978 Sep; 27(5):1061-4. PubMed ID: 717633.
    Abstract:
    Babesia bovis was cultured in a suspension of bovine erythrocytes incubated at 37 degrees C in Medium 199 with 50% bovine serum. The cells in culture were kept in suspension by slow stirring in spinner flasks and the medium was replaced at 24-hour intervals. Persistent multiplication of the parasite in a short series of subcultures suggests the feasibility of this approach for continuous culture.
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