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  • Title: [Isolation and evaluation of properties of muramidase causing the lysis of group A streptococci].
    Author: Shmakova ZF, Savel'ev EP, Chistenkov NA, Petrov GI.
    Journal: Zh Mikrobiol Epidemiol Immunobiol; 1982 Aug; (8):45-50. PubMed ID: 7180263.
    Abstract:
    Muramidase which actively lyses the cell walls of group A hemolytic streptococci has been isolated from the culture fluid of Actinomyces levoris by precipitation on ammonium sulfate, gel filtration on Sephadex G-25, ion exchange chromatography on DEAE cellulose and double electrofocusing. The enzyme thus obtained has shown its maximum activity at 40-50 degrees C. At 60 degrees C muramidase was completely inactivated. The enzyme has a wide range of optimum pH values: 5.0-9.5. The highest percentage of lysis of streptococcal cell walls has been observed in plycine-NaOH and potassium phosphate buffers at pH 8.0. Muramidase completely lysed Streptococcus pyogenes cells of different serological groups except enterococci (group D) and staphylococci. This sign allows to differentiate group D streptococcus from streptococci of other groups.
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