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  • Title: An easy and efficient procedure for the isolation of pure DNA restriction fragments from agarose gels.
    Author: Ledeboer AM, Hille J, Schilperoort RA.
    Journal: Biochim Biophys Acta; 1978 Oct 24; 520(3):498-504. PubMed ID: 718910.
    Abstract:
    A new procedure is developed to isolate DNA from agarose gels. Using a kind of blotting technique, DNA is isolated from the gel. It is shown that the isolated DNA can be used for fragmentation by restriction endonucleases, synthesis of complementary RNA by DNA-dependent RNA polymerase from Escherichia coli and nick translation. The procedure gives a high recovery and is easy to perform.
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