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  • Title: Sodium molybdate increases the amount of progesterone and estrogen receptor detected in certain human breast cancer cytosols.
    Author: Anderson KM, Phelan J, Marogil M, Hendrickson C, Economou S.
    Journal: Steroids; 1980 Mar; 35(3):273-80. PubMed ID: 7189611.
    Abstract:
    When sodium molybdate is added at a final concentration of 20 mM, additional 8S and 4S progesterone (3H-R5020) receptor can be detected in the cytosols from a number of human breast cancers. Additional estrogen receptor also could be measured in some cytosols, and a quantitative temperature-dependent conversion of 8S to 4S binding molecules achieved. Sodium molybdate also prevented the loss of binding activity that occurred when cytosols were incubated at 30 degrees in the absence of added estradiol. In addition to increasing the amount of progesterone receptor, and to a lesser extent estrogen receptor that may be detected, elicidation of the mechanism by which this salt stabilized receptors should contribute to further understanding of how cytosol steroid receptor content and function is regulated. The effect of sodium molybdate (NaMo) on the ability to detect progesterone and estrogen receptors from human breast cancers was studied. When NaMo was added at 20 mM to cytosols from human breast cancers, progesterone receptor showed major increases by sucrose density gradient centrifugation with tritiated R5020 as ligand. Increases in both 8S and 4S receptors, or in 8S alone, also occurred (reported figuratively). When NaMo addition was analyzed by dextran-coated charcoal method, binding also increased, converting some apparently negative cytosols to positive and increasing the amount of receptor in other positive samples. Additional estrogen receptor could also be measured in some cytosols, and a quantitative temperature-dependent coversion of 8S to 4S binding molecules could be achieved. NaMo prevented loss of binding in 30-degree-incubated cytosols in the absence of added estradiol. NaMo increased amount of progesterone receptor, and to a lesser extent estrogen receptor, but how and why are not known.
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