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  • Title: Initiation of transcription in permeabilized Novikoff hepatoma cells.
    Author: Samal B, Ballal NR, Busch H.
    Journal: Cell Biol Int Rep; 1980 Feb; 4(2):175-84. PubMed ID: 7190073.
    Abstract:
    Novikoff hepatoma cells, grown in monolayer cultures, when permeabilized by treatment with lysolecithin, incorporated 3H-UTP at a high rate for 2 hours at 25 degrees. The incorporation was inhibited by initiation inhibitors such as rifampicin AF/013, heparin and aurintricarboxylic acid. About 75% of RNA polymerase II, and 45% of RNA polymerase I activities were inhibited by rifampicin AF/013. In contrast, transcription in isolated nuclei was not inhibited either by rifampicin AF/013 or heparin. The permeabilized cells apparently retain the mechanisms for reinitiation in vitro and may be a useful model for studies on the effects of proteins on gene transcription.
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