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  • Title: Studies on Alternaria allergens. III. Isolation of a mafor allergenic fraction (ALT-I).
    Author: Yunginger JW, Jones RT, Nesheim ME, Geller M.
    Journal: J Allergy Clin Immunol; 1980 Aug; 66(2):138-47. PubMed ID: 7190582.
    Abstract:
    The allergens present in crude Alternaria alternata extract were fractionated by sequential ammonium sulfate precipitation, DEAE cellulose ion-exchange chromatography, preparative flat-bed electrofocusing, and Sephadex G-100 gel filtration. The carbohydrate-rich fraction isolated (Alt-I) was heterogeneous by polyacrylamide gel disc electrophoresis and by thin-layer electrofocusing. By preparative polyacrylamide gel electrophoresis the Alt-I fraction could be further separated into at least five subfractions, all of which contained similar allergenic activity. Significant elevations in IgE antibody to Alt-I were found in 21
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