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  • Title: [Nuclear and cytoplasmic ribonucleoprotein complexes of normal mouse liver cells, Guelstein hepatomas 22A and 48 and of liver of tumor-bearing animals].
    Author: Alekhina RP, Moiseev VL, Zborovskaia IB.
    Journal: Biokhimiia; 1978 Sep; 43(9):1710-7. PubMed ID: 719074.
    Abstract:
    Nuclear and cytoplasmic RNP complexes obtained from normal mouse liver cells, Guelstein hepatomas of different degrees of malignancy (22A and 48) as well as from liver of tumor-bearing mice were subjected to chromatography on a celite column (NPC--chromatography). In addition cytoplasmic RNP complexes were centrifuged in sucrose and CsCl density gradients. The results of the NPC-chromatography indicate that nuclear rapidly labelled RNA species of all tissues under study are constituents of the two main types of RNP particles differing from each other by the tightness of RNA-protein bonds. No precursor-product relationship could be revealed between the above types of RNP-particles of nucleus, labelled under conditions of a partial Actinomycin D block. Rapidly labelled nonribosomal cytoplasmic RNAs represent constituents of RNP-particles resembling nuclear ones in their degree of heterogeneity and chromatographic position. Sedimentation analysis of cytoplasmic RNP-particles from tumours showed an increase in relative proportion of monoribosomes and informosomes (free non-ribosomal cytosol RNP-complexes) at the expense of polyribosomes and mRNP complexes. Thus, the liver cells of experimental tumour-bearing animals undergo changes (although not very well-defined), typical for tumour cells.
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