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  • Title: Demonstration of sulfhydryl and disulfide groups by a fluorescent maleimide procedure.
    Author: Curtis SK, Cowden RR.
    Journal: Histochemistry; 1980; 68(1):23-8. PubMed ID: 7190963.
    Abstract:
    Several fluorescent maleimide compounds were evaluated as possible substitutes for N-(4-aminophenyl)maleimide in the histochemical procedures developed by Sippel (1973, 1978a, b, 1980) for the demonstration of sulfhydryl and disulfide groups. The brightest and most selective fluorescence was obtained by using N-(7-dimethylamino-4-methylcoumarinyl)maleimide (DACM), although both eosin-5-maleimide and fluorescein-5-maleimide could also be used if adequate control preparations were made.
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